8.1 Determination of neutral detergent fibre (NDF)
Apparatus
1) Refluxing apparatus: Tall pyrex or corning beakers (spout less) of about 500 ml capacity round bottom flask as condenser
2) Sintered glass crucibles with coarse porosity (Grade 1) of about 50 ml capacity
3) Electronic balance
4) Vacuum pump
5) Hot plate
6) Wash bottle
7) Hot air oven and muffle furnace
Reagents
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Neutral detergent solution: |
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Distilled water |
1 litre |
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Sodium lauryl sulphate |
30 g |
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Disodium ethylene di-aminetetraacetate (EDTA) dehydrate |
18.61 g |
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Sodium borate decahydrate |
6.81 g |
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Disodium hydrogen phosphate (anhydrous) |
4.56 g |
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2-ethoxyethanol (ethylene glycol monoethyl ether) |
10 ml |
